Speaker: Mustafa DEMİRTAŞ, PhD
Title: Pushing the limits of biosensors and bioelectronics: Detection of biomarkers with single-cell specificity and single-molecule precision using nanopipettes
Date & Time: 01.11.2021, 20:00
Location: Microsoft Teams 

Abstract: Due to the lack of analytical techniques for detecting biomarkers near living cells, developing tools for in situ cellular analysis of single living cells and detection of secretomes remains a challenge to overcome in bioelectronics. The secreted biomarkers play a pivotal role in cancer progression and metastasis by orchestrating migration and invasion.  Thus, the secretomes may offer signatures of a metastatic phenotype and in removing the veil on the communication mechanisms of the same or different types of cells.  But it is challenging to detect them since the biomarkers are secreted only in minute amounts and then diluted in blood plasma or contaminated by a cell culture medium. In this work, we have developed an approach to detect the secretomes from different types of cells such as cancer cells, stem cells, and neurons based on ionic and blockade current distribution measurement through a nanopipette biosensor. Thus, it could be someday a new tool for the molecular diagnosis of cancer has been validated that can measure aspects of the secretome with single-cell specificity and single-molecule precision.